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KMID : 0380020130280030208
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 3 p.208 ~ p.212
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Effect of Pore Size of Mesoporous Spherical Silica for the Purification of Paclitaxel from Plant Cell Cultures
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Oh Hyeon-Jeong
Jung Kyeong-Youl
Kim Jin-Hyun
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Abstract
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Four types of mesoporous spherical silica adsorbents with different physical properties were prepared by spray pyrolysis and were used for the purification of the anticancer agent paclitaxel from plant cell cultures. Pore size had a greater effect on the removal of plant-derived impurities during the pre-purification of paclitaxel compared with surface area and pore volume. An appropriate pore diameter (~9.07 nm) was required to achieve the highest purity (~46.1%) and yield (~82.3%) of paclitaxel. These results were confirmed by HPLC analysis of the absorbent after treatment and Thermogravimetric analysis of the organic substances bonded to the adsorbent.
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KEYWORD
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Paclitaxel, Purification, Mesoporous silica, Adsorbent, Pore size
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